Structure of the Intermolecular Complex between Plastocyanin and Cytochrome f from Spinach*
نویسندگان
چکیده
منابع مشابه
Chapter IV Paramagnetic NMR using spin-labeled proteins to study the structure of the complex between cytochrome f and plastocyanin
متن کامل
Hydrophobic interactions in a cyanobacterial plastocyanin-cytochrome f complex.
The complex of the photosynthetic redox partners plastocyanin and cytochrome f from the thermophilic cyanobacterium, Phormidium laminosum, was investigated by nuclear magnetic resonance (NMR). Chemical-shift perturbation analysis of amide proton and nitrogen nuclei implicates the hydrophobic patch and, to a lesser extent, the "eastern face" of plastocyanin in the complex interface. Intermolecul...
متن کاملThe Complex of Cytochrome f and Plastocyanin from Nostoc sp. PCC 7119 is Highly Dynamic
Cytochrome f (Cyt f) and plastocyanin (Pc) form a highly transient complex as part of the photosynthetic redox chain. The complex from Nostoc sp. PCC 7119 was studied by NMR relaxation spectroscopy with the aim of determining the orientation of Pc relative to Cyt f. Chemical-shift-perturbation analysis showed that the presence of spin labels on the surface of Cyt f does not significantly affect...
متن کاملThe cytochrome f-plastocyanin complex as a model to study transient interactions between redox proteins.
Transient complexes, with a lifetime ranging between microseconds and seconds, are essential for biochemical reactions requiring a fast turnover. That is the case of the interactions between proteins engaged in electron transfer reactions, which are involved in relevant physiological processes such as respiration and photosynthesis. In the latter, the copper protein plastocyanin acts as a solub...
متن کاملDynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica.
The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2005
ISSN: 0021-9258
DOI: 10.1074/jbc.m412760200